|
KMID : 0613820090190070963
|
|
Journal of Life Science
2009 Volume.19 No. 7 p.963 ~ p.967
|
|
|
Neuroprotective Effect of Extracts from Root Bark of Morus alba on Glutamate-induced Cytotoxicity in Neuronal Cells
|
|
Kim Hyun-Jung
Kim Ji-Hyun
Son Eun-Soon
Lee Jung-Min
Park Hae-Ryong
|
|
|
Abstract
|
|
|
|
This study evaluated the neuroprotective effect of extracts from the root bark of Morus alba (MA) against glutamate-induced cytotoxicity in neuronal cells. Glutamate-induced cytotoxicity was shown by MTT reduction assay. The neuroprotective effects of methanol, ethanol, and acetone extracts from MA against glutamate-induced cytotoxicity were measured. Among the three extracts, the methanolic extracts showed the highest protective effect, as determined by the results of an morphological assay, a lactate dehydrogenase release assay. Furthermore, the methanol extracts were fractionated sequentially with hexane, diethyl ether, ethyl acetate, and water layer according to degree of polarity. The hexane fractions exhibited a neuroprotective effect against glutamate-stressed N18-RE-105 cells. Therefore, these results suggest that extracts of MA could be a new potential candidate as a protective substance against glutamate-induced cytotoxicity.
|
|
|
KEYWORD
|
|
|
Neuroprotective effect, Morus alba, glutamate, cytotoxicity, N18-RE-105 cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|